Ultrastructural markers of tissue endometrial receptivity in patients with recurrent implantation failure

Abstract

The scanning electron microscopy of the endometrial surface epithelium during the ‘implantation window’ was performed in 119 patients with uterine factor of infertility or recurrent miscarriage due to endometrial hypoplasia. Ultramorphological picture of the surface endometrial epithelium was characterized by aplasia and hypoplasia of pinopodes (67.39%), dense cell – cell contacts (69.53%), heteromorphy of secretory cells (15.22%) in combination with atypia of microenvironment cells (50%) in patients with infertility. The asynchronous development of pinopodes (46.67%) and the absence of intercellular contacts separation during the ‘implantation window’ (84.44%) was observed in patients with recurrent miscarriage. The revealed disturbance determines the mechanisms of the blastocyst adhesion violation and trophoblast invasion in the different stages of implantation in patients with uterine factor of infertility and recurrent miscarriage.     

分析预防子宫内膜癌术后下肢深静脉血栓形成的护理干预效果

目的 探讨预防子宫内膜癌术后下肢深静脉血栓形成的护理干预措施及效果。方法 50例子宫内膜癌患者,按照护理方式不同分为研究组和对照组,各25例。对照组实施常规护理,研究组实施综合护理。对比两组患者术后下肢深静脉血栓形成率及生活质量评分。结果 研究组患者术后下肢深静脉血栓形成率0明显低于对照组的32.0%,差异具有统计学意义(P<0.05)。研究组患者活动能力、临床症状、日常生活质量评分及总分分别为(84.6±3.6)、(89.4±4.2)、(92.0±1.2)、(93.3±2.5)分,均明显高于对照组的(73.4±5.1)、(78.3±3.6)、(80.6±4.1)、(79.6±4.9)分,差异均有统计学意义(P<0.05)。结论 子宫内膜癌患者术后实施综合护理能够有效提升护理质量,降低术后下肢深静脉血栓形成率,缓解患者术后各类不适症状,提升患者术后生活质量,值得推广应用。     

骨髓腔穿刺输液在院外创伤失血性休克患者抢救中的应用效果

目的比较通过骨髓腔输液与常规静脉输液对于院外创伤失血性休克患者抢救应用效果。方法选取2017年7月-2018年7月北京急救中心中区分中心院外救治的创伤失血性性休克患者100例随机分骨髓腔穿刺组(IO,n=50)和常规静脉穿刺组(IV,n=50)。分别应用骨髄腔穿刺或常规静脉穿刺进行快速补液治疗。比较建立补液通道所用的时间,一次性穿刺成功率,现场救后生存率及不良反应。结果骨髓腔穿刺组建立有效补液通道时间明显比常规辞脉穿刺组短(P<0.05),骨髓腔穿刺组一次穿刺成功率明显高于常规静脉穿刺组(P<0.05),骨疏腔穿刺组现场救后生存率明显高于常规静脉穿刺组(P<0.05),骨髓腔穿刺组发生液体渗漏的患者明显少于常规静脉穿刺组(P<0.05)。结论常规静脉穿刺对救治环境和患者自身条件有一定的要求,院外现场急救因其特殊性有时难以保证常规静脉穿刺的顺利进行。采用骨髓腔穿刺建立补液通路具有操作简单、快捷,输液通路穩定性高,方便及时给予抢救药物,可为创伤失血性休克患者的抢救争取宝贵的时间。骨髓腔穿刺具有一次穿刺成功率高,操作时间短,不良反应少等优点,值得在院外现场抢救中推广应用。     

Inhibitory neuronal changes following a mixed diffuse-focal model of traumatic brain injury

Traumatic brain injury (TBI) can result in excitation: inhibition imbalance, as well as a range of chronic neurological deficits. However, how TBI affects different interneurons, and how this relates to behavioral abnormalities, remains poorly understood. This study examined the effects of a mixed diffuse-focal model of TBI, the lateral fluid percussion injury (LFPI), on interneurons, 8 weeks post-TBI in rats. Brains were labeled with antibodies against calbindin, parvalbumin, calretinin, neuropeptide Y, and somatostatin, and the number of interneurons were assessed in the cortex and hippocampus following LFPI. LFPI caused a reduction in the numbers of interneurons mediating both perisomatic and dendritic inhibition in the somatosensory cortex. In hippocampus, there were heterogenous changes in the number of interneurons while motor cortex, showed no obvious loss in any of the subsets of interneurons after TBI. In parallel to the investigations of changes in the number of interneurons, we also investigated the long-term behavioral consequences of LFPI. Behaviorally, rats given an LFPI displayed transient reduction in performance in motor tasks and were significantly impaired in reversal learning in the water maze task post-TBI. We also report here progressive neurodegeneration in cortex and hippocampus indicated by Fluoro-Jade C in the different brain areas examined after injury. Our findings suggest differential vulnerability of inhibitory neurons to LFPI in the different brain areas examined after injury. These data will aid in evaluation of new treatments for TBI and help target specific neuronal subtypes as a function of injury time and type.